《植物生理学报》 2018, 54(8): 1349-1355

新塔花的组织培养与快速繁殖

马丽娜¹, 何江^1,2, 李冠^1,*

¹新疆大学生命科学与技术学院, 乌鲁木齐830046; ²新疆药物研究所, 乌鲁木齐830004

通信作者：李冠；E-mail: guanli@xju.edu.cn

摘　要：

以新疆特有药用植物新塔花(Ziziphora bungeana Juz.)幼嫩茎段为外植体材料, 筛选初代培养、继代增殖与生根培养的条件, 建立了新塔花组织培养与快速繁殖体系。结果表明: 新塔花种子最适萌发培养基为MS+0.25 mg·L^-1 NAA, 萌发率达57.78%; 茎段诱导腋芽最适培养基为MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA, 诱导率为90.47%; 适宜芽增殖培养基为MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+0.3 mg·L^-1 GA₃+35 g·L^-1蔗糖, 20 d增殖系数达1.95; 最佳诱导根生长培养基为1/2MS+0.5 mg·L^-1 NAA+0.2% AC, 生根率达60%, 平均生根数为3.5, 20 d株高达4.5 cm。移栽至混合基质(营养土:珍珠岩:蛭石=1:1:1)中, 组培苗成活率为80.45%。

关键词：新塔花; 组织培养; 快速繁殖; 茎段

收稿：2018-06-22   修定：2018-07-16

资助：新疆维吾尔自治区天山创新团队计划(2017D14014)。

Tissue culture and rapid propagation of Ziziphora bungeana Juz.

MA Li-Na¹, HE Jiang^1,2, LI Guan^1,*

¹College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; ²Institute of Materia Medic, Xinjiang, Urumqi 830004, China

Corresponding author: LI Guan; E-mail: guanli@xju.edu.cn

Abstract:

The tissue culture and rapid propagation system of Ziziphora bungeana Juz. was established by utilizing its healthy tender stem as explant. We analyzed affecting factors of culture medium of hormone and additional nutrient for axillary bud induction, subculture proliferation and rooting culture. The results showed that the suitable medium for germination of seeds was MS supplemented with 0.25 mg·L^-1 NAA, the germination rate was 57.78%. The optimal medium for stem segments inducing adventitious buds was MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA, with an induction rate of 90.47%. The suitable combination of bud proliferation was MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+0.3 mg·L^-1 GA₃+35 g·L^-1 sucrose. The multiplication coefficient was 1.95 after 20 days. The most optimum rooting medium was 1/2MS+0.5 mg·L^-1 NAA+0.2% AC, with a rooting rate of 60%, the average rooting numbers of 3.5 and plant length of 4.5 cm for 20 days. Plantlets were transplanted to a potting mixture (1:1:1, nutritive soil: perlite: vermiculite) in trays with about 80.45% survival percentage.

Key words: Ziziphora bungeana Juz.; tissue culture; rapid propagation; stem segment